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Expert in Applied Biochemistry, Microbiology
Available for your Consulting and Expert Witness Needs
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Summary of Expertise:
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Listed with other top experts in: |
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Expert has spent his entire professional career at the forefront of applied microbiology and biochemistry. Using the most advanced techniques of molecular biology, he has been devoted to isolating, characterizing, and studying the reaction mechanisms of enzymes which catalyze unusual and interesting reactions, like the insertion of halogen and oxygen atoms into organic molecules.
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Expert's work requires specific expertise with regard to a variety of subjects including amino acids, peptides, proteins, chemotherapeutic agents, antibody production, whole cell cultures, and gene regulation. Expert has five years experience in industry. He has served on the National Science Chemistry Advisory Board and has served as a consultant to the National Science Foundation and the N.I.H. He has served as a member of the Scientific Advisory Board of a Biotechnology Company. He is an elected member of the National Academy of Sciences.
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Chloroperoxidase, an enzyme which uses H2O2 and catalyzes halogenation and epoxidation reactions, was discovered in Expert's laboratory many years ago. Subsequent research has involved studies on the mechanisms of the reactions and the utility of chloroperoxidase in the synthesis of chiral epoxides. The gene for chloroperoxidase has been cloned. Current efforts are devoted to site specific mutagenesis in order to extend stereoselectivity to halogenation reactions and to expand the number of organic acceptor molecules which function in halogenation and epoxidation reactions. Using "directed evolution" techniques the Expert lab has produced chloroperoxidase mutants that can function in the presence of high concentrations of organic solvents. A second directed evolution mutant is resistant to mechanism based inactivation during the epoxidation of primary olefins.
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Halophytic plants synthesize and distill methyl chloride into the atmosphere. Expert's interest has been "how and why" plants carry out this reaction. He has isolated and cloned an enzyme which catalyzes the transfer of a methyl group from S-adenasye methionine to chloride ion. This enzyme answers the question "how." He is attempting to answer the question "why." His basic hypothesis is that halophytic plants control their internal chloride ion concentrations by making a volatile methyl chloride. He is attempting to clone the transferase gene and transform soybean plants to enable them to make methyl chloride. He the plans to compare wild-type and transformed soybeans in their ability to grow under high salt environments.
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Show Secondary and Basic Areas of Expertise | Localities:
Expert may consult nationally and internationally, and is also local to the following cities: Peoria, Illinois;
Springfield, Illinois;
Joliet, Illinois;
Decatur, Illinois;
Champaign, Illinois;
Bloomington, Illinois;
Terre Haute, Indiana;
and Lafayette, Indiana.
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Degree |
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Subject |
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Institution |
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1953
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Ph.D.
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Microbiology
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(Undisclosed University)
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1950
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M.A.
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Microbiology
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University of Kansas
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1947
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B.A.
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Chemistry and Biology
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Valparaiso University
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| Years |
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Employer |
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Title |
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1987 to
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(Undisclosed University)
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Director, Biotechnology Center
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1960 to
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(Undisclosed University)
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Professor of Biochemistry
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1955 to 1960
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Harvard University
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Assistant Professor of Biochemistry
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| Language Skills: |
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| Foreign Language |
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Description |
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German
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Expert can read German.
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French
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He can read French.
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Education
